Proteomics

We provide investigators access to advanced mass spectrometry instrumentation and analysis for protein identification, characterization, and quantification.

Services

Our facility provides comprehensive proteomic analysis services using state-of-the-art equipment, including:

  • Sample preparation
    • We accept a wide range of samples from simple proteins to complex tissues and offer all sample handling and protein extraction services to properly prepare samples for downstream analysis. Including; cell lysis, protein precipitation, protein quantitation, sample clean-up, digestion, and enrichment.
  • Protein identification
    • We provide identification(s) for protein or a mixture of proteins using bottom-up proteomics approaches. Samples are enzymatically digested, analyzed with nano LC-MS/MS; using either DDA (data-dependent acquisition) or DIA (data-independent acquisition) approaches. Data are then searched enabling the proteins to be identified.
  • Posttranslational modification analysis
    • Common PTM analysis includes phosphorylation, ubiquitination, methylation, acetylation and oxidation. Samples may be enriched prior to digestion and then analyzed by LC-MS/MS to determine the sequence of the peptide and the location of the modification. In addition, we have extensive experience working with researchers to identify and characterize customized post-translational modifications and amino acid mutations.
  • Protein quantitation
    • We offer several approaches to monitor differentiation in protein expression between samples, including label free approaches (spectral counting), TMT (tandem mass tag), and SILAC (stable isotope labeling with amino acids in cell culture).
  • Protein-protein interactions
    • We routinely perform bottom-up proteomics on co-immunoprecipitation experiments to identify physiologically relevant protein interactions.
  • Intact protein and protein complex analysis
    • We also offer the analysis of intact proteins and protein complexes using native mass spectrometry approaches (including online buffer exchange-native MS). Which enables the intact mass to be determined of covalent and non-covalent complexes including protein:protein, protein:RNA/DNA, protein:ligand complexes.

 

State-of-the-art instrumentation

Our proteomics instrumentation includes:

  • Bruker timsTOF Pro
  • Thermo Orbitrap Eclipse
  • Thermo Orbitrap Fusion
  • Thermo Q Exactive plus
  • Thermo Q Exactive UHMR (for native MS experiments)

Additional Information

Schedule a meeting with our proteomics team to help design and implement your research project. Please contact ccic-msp@osu.edu
Mass Spectrometry and Proteomics