Instruments

Ultrahigh resolution (>1,000,000) and mass accuracy (< 1 ppm). Equipped with a dual ionization source (MALDI and ESI). ETD and ECD options available. Used mostly for accurate mass analysis, complex mixture analysis, untargeted metabolomics, and small molecule MALDI imaging. The 15 T Bruker SolariXR FT-ICR instrument was supported by NIH (S10 OD018507).

Ultrahigh resolution and excellent mass accuracy tribrid mass spectrometer. This instrument is equipped with CID, HCD, and ETD and is coupled with a nanoflow HPLC.  Used mostly for bottom-up proteomics. The Fusion Orbitrap instrument was supported by NIH (S10 OD018056).

Ultra high resolution and excellent mass accuracy tribrid mass spectrometer.  This instrument is equipped with CID, HCD, UVPD and ETD and is coupled with a nanoflow HPLC.  Used mostly for bottom-up proteomics. It is also equipped with proton transfer charge reduction and an extended mass range useful for native mass spectrometry. 

High resolution TOF with trapped ion mobility prior MS/MS fragmentation. The instrument has high duty cycle (almost 100%) and high sensitivity, requiring injections of only 200ng. Used exclusively for bottom-up proteomics studies. This instrument is coupled with a nanoflow HPLC. The timsTOF Pro instrument was supported by NIH (S10 OD026945).

Ultrahigh resolution and excellent mass accuracy. Equipped with protein mode and a nano LC. Used for bottom-up proteomics and native mass spectrometry studies.

Ultrahigh resolution and excellent mass accuracy. This QE plus is exclusively used for untargeted and targeted metabolomics.

Ultrahigh resolution and excellent mass accuracy. Equipped with ESI and APCI. Used for untargeted and targeted metabolomics. The Exploris 480 was supported by the OSU Comprehensive Cancer Center’s Intramural Research Program (OSUCCC IRP)

An ultrahigh performance triple quadrupole LC-MS/MS instrument. Equipped with the latest iFunnel technology, this LC/MS/MS achieves ppq-level detection limits. This instrument is used exclusively for targeted compound quantification. The Agilent 6495D was supported by the OSU Comprehensive Cancer Center’s Intramural Research Program (OSUCCC IRP)

The Bruker-Daltonics UltrafleXtremeMALDI-TOF-TOF MS-Tissue Imaging Platform with collision-induced dissociation (CID) capabilities for structural determination by MALDI. The instrument was supported by the OSU Comprehensive Cancer Center’s Intramural Research Program (OSUCCC IRP).

Equipped with a dual ionization source (MALDI and ESI), trapped ion mobility separation, and a MALDI-2 post-ionization laser to enhance sensitivity and range while decreasing ionization suppression. Reaching spatial resolutions of 5-10 micrometers, this instrument represents cutting-edge technology for tissue and cellular mass spectrometry imaging.