Sophie Harvey, PhD

I am the Associate Director of the CCIC Mass Spectrometry and Proteomics facility as well as the NIH RM1 funded native MS Guided Structural Biology Center. I work on proteomics and native mass spectrometry samples within the Ohio State Mass Spectrometry and Proteomics Facility. My work on native mass spectrometry involves studying intact protein-protein, protein-ligand, and protein-RNA/DNA complexes which can be used to determine stoichiometry and relative affinities. In addition, I use bottom-up and top-down proteomics approaches to identify proteins, post translational modifications, and for relative quantitation. My work mostly uses the Thermo Orbitrap Fusion, Thermo Q Exactive Plus, Thermo Orbitrap Eclipse, Bruker timsTOF Pro, and the 15T FT ICR MS. My role also includes data analysis of these samples using a variety of different software packages-including Mascot and Proteome Discoverer. 

Prior to working in the facility I received my PhD from the University of Edinburgh working under the supervision of Perdita Barran, using MS and ion mobility-MS to study conformationally dynamic proteins and their interactions with small drug molecules or biomolecules. Following this I did postdoctoral research in the lab of Vicki Wysocki at The Ohio State University studying the structure of protein complexes, and membrane protein complexes, using MS and IM-MS.