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Mass Spec and Proteomics > About Us

Welcome to the Campus Chemical Instrument Center (CCIC) Mass Spectrometry and Proteomics Facility at The Ohio State University (OSU)

We hope you will find that we can meet your research needs as we offer a wide range of services. Please feel free to contact us to discuss the wide range of experiments we can perform.

CCIC MSP Lab - 2019

The mandate of the CCIC Mass Spectrometry and Proteomics (MSP) Facility is to provide state-of-the-art instrumentation and personnel expertise for a wide variety of research projects. Main areas of services we provide include i) proteomics, ii) metabolomics, and iii) general mass spec analyses for research groups. We also participate and provide support for grant applications and education. The Mass Spectrometry and Proteomics Facility at CCIC is an interdisciplinary unit, servicing faculty from the colleges of Biological Sciences, Education and Human Ecology, Engineering, Food, Agriculture & Environmental Sciences, Mathematical and Physical Sciences, Medicine, Optometry, Pharmacy, and Veterinary Medicine just to name a few. CCIC also serves scientists from other universities and industry within and outside of Ohio.

Please Note

The Mass Spectrometry & Proteomics Facility CANNOT ACCEPT RADIOACTIVE or HAZARDOUS MATERIALS

In order to minimize contamination in mass spectrometry and proteomics it is recommend you review this PDF prior starting your experiment(s).

Instructions for submitting samples to the Mass Spectrometry & Proteomics Facility:

  1. If you do not know what kind of service you need, please send an email to ccic-msp@osu.edu.
  2. Submit a Service Request Form through FOM – see instructions on Rates & Forms page of our website.
  3. Please provide as much information as possible regarding the sample and desired analysis. Please note any special sample instructions or analysis instructions on the form.
    The more information we have, the better the results and the faster we can return data.
  4. Bring sample(s) and the Service Request Form between 8AM & 5PM or mail to:
                    OSU Mass Spectrometry & Proteomics Facility
                    460 W 12th Ave, Rm 250 Biomedical Research Tower (BRT)
                    Columbus, OH 43210
                    Phone: 614-247-8789   
  5. Bring your Buck ID for access to our Facility in the Biomedical Research Tower.
  6. Results are emailed and all data is electronic.
  7. Samples are discarded after analysis, so please indicate if you would like to pick up any unused sample.

Sample Submission Guidelines

Samples can be brought to 250 Biomedical Research Tower, along with a copy of your service request form, weekdays between 8 am and 5 pm. We have a refrigerator, freezer and dry box for samples that require special storage. It is preferred if samples are submitted neat or dried down, typically ug amounts is all that is needed.

Biological samples are handled differently and require different conditions. Salts, buffers and surfactants suppress ionization and need to be removed for mass spec analysis. When possible, it is advised to dialyze the sample into nanopure or at least distilled water prior to submission. We also have several de-salting techniques for proteins and oligonucleotides available in our facility. List all salts, buffers and detergents the protein or oligonucleotide is in, or has recently been in. For optimal results, please discuss your project with CCIC MSP research staff prior to submission. Certain classes of proteins need to be handled differently than others, the more we know about your system, the better the data. Protein samples for ESI are prepared in 50% acetonitrile 50% water with 0.1% formic acid. Slightly acidic conditions are necessary to facilitate protonation

We CANNOT run samples that are in aromatic solvents like benzene, toluene, or solvents such as DMSO and DMF.

Electrospray and MALDI samples are prepared in Methanol, Acetonitrile, water, THF, chloroform. We will acidify the sample with acetic or formic acid unless you indicate the sample is acid sensitive.

Turn around times vary between 1 day to a week depending on sample load, instrument availability and type of analysis. We will email you when the results are ready.